Original Data

Bis(quercetinato)oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice

Ruchi Shukla¹, Subhash Padhye², Manisha Modak³, Saroj S. Ghaskadbi³, Ramesh R. Bhonde¹

Keywords: vanadium, pancreatic regeneration, insulin mimetic, oxidative stress, glucokinase and G-6-Pase

Abstract

Organic vanadium compounds offer several advantages in the treatment of diabetes, yet they are impractical to use because of known side effects. In order to ameliorate the side effects of vanadium, we conjugated it with quercetin to form bis(quercetinato)oxovanadium IV (BQOV). This study evaluates the effect of BQOV treatment on carbohydrate metabolism and overall oxidative stress in streptozotocin-induced (STZ) diabetic mice. Administration of BQOV orally to diabetic mice for 3 weeks led to a reduction of blood glucose levels and the animals exhibited normal glucose tolerance at the end of the study period. The increase in glucose uptake by skeletal muscle and liver as well as the normalization of mRNA levels of G-6-Pase and glucokinase in the liver after BQOV treatment pointed to improvements in carbohydrate metabolism. The analysis of the antioxidant status of serum, liver and pancreas revealed reduced oxidative stress in BQOV-treated animals compared to untreated diabetic controls. Serum analyses for kidney and liver function showed that BQOV treatment provoked total protection of the kidney and partial protection of the liver from diabetogenic insults. The number of insulin-positive cells and the amount of pancreatic insulin in treated mice (1.2038 ± 0.34 ng/mg tissue) did not account for pancreatic regeneration but suggested an insulin-mimetic action on the part of BQOV. Moreover, administration of BQOV for 3 weeks did not show any visible side-effects. This data indicate that BQOV is a safe and potent agent for diabetes treatment, because it is able to improve carbohydrate metabolism and to reduce overall oxidative stress.

Fulltext: HTML , PDF (459KB)

This article has been cited by other articles:

	Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress Zhao Y, Ye L, Liu H, Xia Q, Zhang Y, Yang X, Wang K J Inorg Biochem 2010. 104(4):371-378
	Teucrium polium Complex with Molybdate Enhance Cultured Islets Secretory Function Mohseni Salehi Monfared SS, Pournourmohammadi S Biol Trace Elem Res 2009. Jun 17, epub ahead of print
	Quercetin and green tea polyphenols inhibit the mitochondrial damages and cytotoxicity induced by VO(acac)2 Zhao YB, Shi Z, Ye LH, Liu HX, Yang XD, Wang K J Chin Pharm Sci 2009. 18(3):225-231
	Research progress in anti-diabetic effects of vanadium compounds Wei D, Ding WJ, Zhou J, Peng D, Li M Prog Chem 2009. 21(5):896-902